© BrainNet Europe II
BrainNet Europe is a "Network of Excellence" funded by the European Commission in the 6th Framework Program "Life Science" (LSHM-CT-2004-503039). It consists of 19 established brain banks across Europe and is coordinated by the Centre for Neuropathology and Prion Research Ludwig-Maximilians-University Munich, Germany.
|WP17 - RNA Preservation|
Post-mortem brain and spinal cord tissue is increasingly being used for studying gene expression in neurological and neuropsychiatric diseases. Post-mortem conditions represent a potential source of significant variation amongst samples and previous studies have shown that RNA preservation is not directly related to post-mortem delay time.
The objectives of this work package are to determine the optimal methods for preparation of tissue and extraction and storage of RNA from post-mortem brain and spinal cord tissue such that the RNA preparations are optimal for analysis via a number of techniques, including expression arrays.
The specific objectives are to determine the best methods for tissue preparation and RNA isolation and to determine the factors that affect quality and yield of RNA from human post-mortem tissue. The suitability of RNA isolated using the different methods will be tested for a number of commonly used molecular techniques. The participant laboratories within the consortium will then be trained in the techniques, reagents disseminated, and a standard practice developed for the supply of frozen human neural tissue for post-genomic era research throughout Europe.
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