Budapest HBTB, Hungary
Human Brain Tissue Bank Budapest

Participant 14: Human Brain Tissue Bank Budapest
Tüzolto u. 58
1094  Budapest
Hungary

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Project Leader


Prof. Miklós Palkovits
Phone: +36 (1) 2160 488  
Fax: +36 (1) 2181 612  
 
Prof. Miklós Palkovits


Project Staff


Dr. Katalin Gallatz
Assoc. professor 

Zsolt Kovács
Assoc. professor 

Kinga Meltzer
Biologist  

Rege Sugárka Papp
Biologist   

Nora Nora
Biologist   

Balázs Balázs
PhD fellow  

Eszter Kirilly
PhD fellow  

Viktoria Bencze
Doctoral Student  

Attila Trájer
Doctoral Student  

Judith Máté
Doctoral Student 

Judith Helfferich
Technician  

Technician Technician
Technician 

Maria Tóth
Technician   


Institute Presentation


Summary of experience relevant to brain banking

The Human Brain Tissue Bank (Budapest) may provide microdissected human brain samples (up to 20000 samples - at the present time) suitable for neurochemistry and molecular biology. Three types of dissections are performed:

a) cut out topographically oriented brain areas as about 1 x 1 x 2 cm tissue blocks ready for slicing in a cryostat. These samples are excellent for in situ hybridisation histochemistry, localization mRNAs or labelled substances on tissue sections.

b) From soma brains, relatively large areas (tissue weights between 0.1 and 2 g) area dissected, called "macrodissection". In that case, 30 different brain samples represent the whole brain both functional and morphological points of view.

c) The "microdissected" brain samples represent the major collection of our Tissue Bank having nearly 20000 samples. We use the "micropunch" technique to remove more than 100 different, individual nuclei or areas from a single brain. These samples are improved to be excellent for neurochemical analyses and microassays. Microdissected brain tissue samples from persons died neurological and neurodegenerative diseases are also collected and will be microdissected together with brains from other European Human Brain Banks.

Research with relevance to topics of the network

Special point of the microdissection of the human brains:

1) Very short post mortem delay. Most of the brains were removed from the skull and frozen within 2 hours after death. These brains were from the Department of Forensic Medicine of the Semmelweis University from persons with sudden death in public places or died in traffic accidents. Additionally, we have brains of suicide victims and some brains from those who died in neurological diseases with somewhat longer (2-6 hours) post mortem delay.

2) We have brain samples (mainly from the cerebral cortex) with zero hour post mortem delay, i.e. tissue samples were taken during neurosurgery and frozen on dry ice right in the surgical room.

3) We do not store whole brains or large brain pieces, all of our samples are microdissected. The whole procedure is done on frozen brains and the samples are kept on -70°C. We are ready to collaborate with the other Human Brain Banks and perform - if it is requested - microdissections on their brains or brain pieces. During the past 30 years, I have learned and practiced human brain microdissections for up to 180 different brain regions including 47 different cerebral cortical (Broadmann) areas. These samples are improved to be excellent for protein determination, neurochemical analyses and microassays. The number of scientific collaborators using samples from the HBTB in the past 10 years is 27. The number of scientific publications in international journals based of tissue samples from the HBTB in the past 10 years is 42.




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