WP23 - Laser Capture Microdissection

The applicability of high quality human post mortem human tissue to new sampling techniques such as laser capture microdissection (LCM), were investigated on the basis of results of WP01, WP17, WP18, WP19 and WP20. The limitations and possibilities of this innovative technique using human post mortem brain tissue are being determined, both for RNA and protein isolation/detection. This sampling technique may be given even more specific when it is coupled with histochemical or immunohistochemical stainings. The specific objectives are: 

  • To determine the best methods for tissue preparation and RNA or proteins isolation
  • To determine the factors that affect the quality of RNA and protein yields from human post-mortem brain tissue when using some histochemical and immunohistochemical stainings.
  • To assess the suitability of RNA and proteins isolated using LCM for a number of commonly used molecular techniques such as gene expression arrays, real time PCR and mass spectrometry. 
  • To determine of the minimum amount of tissue areas/cells or structures of interest (for example amyloid-plaques in Alzheimer's disease) required for those different sophisticated molecular techniques.
  • To compare the different existing models of laser capture microscopes.

Workpackage Leader

Dr. David Meyronet

Phone: +33 (4) 7235 7630 
Fax: +33 (4) 7235 7067  
Dr. David Meyronet

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